Reading & Resources — Week 2

Core

• DNA Sequencing at 40: Past, Present, and Future (2017) — Shendure, Balasubramanian, Church, et al. Nature
• DNA Synthesis Technologies to Close the Gene Writing Gap (2023) — Hoose, Vellacott, Storch, et al. Nature Rev Chem
• Recombineering and MAGE (2021) — Wannier et al. Nat Rev Methods Primers
• CRISPR: A Decade of Genome Editing is Only the Beginning — Wang, Doudna, et al. Science

Databases

• GenBank overview — NCBI
• NCBI Genome — NCBI
• Ensembl — Ensembl
• UCSC Genome Browser — UCSC | Harvard GMC

Editors & Tutorials

• CRISPR/Cas9 — Addgene tutorial | Benchling guide | PAM sequences
• Base editors — Review (2018)
• Prime editing — Prime editor | primeedit.nygenome.org | guide
• TALENs — Short guide | Design resource | Directed evolution

Additional Resources

• Gel purification of DNA — Addgene
• Synthetic genomes with altered genetic codes (2020) — ScienceDirect
• DNA recorders — Nature (2021)
• Single‑molecule sensors on chips — PubMed (2022)
• Genome editors review (pre‑CRISPR era) — Trends Biotech (2013)
• Clinical trials of genome editing therapies — Nature (2020)