Lab — Gibson Assembly
Tip
This week’s lab pairs directly with the Homework. Use the protocol and primer‑design notes below; document your process and results.
Objective
Practice DNA assembly by preparing linear fragments (via PCR and/or restriction digest) and assembling them with Gibson Assembly, followed by transformation into E. coli.
Concepts You’ll Learn
- Primer design fundamentals
- PCR setup and thermocycling
- Restriction digests & buffer selection
- Gibson Assembly principles & workflow
- Transformation and selection
Protocol & Prep
