Lab — Cell Free Induction
Info
MIT/Harvard: No in‑person lab protocol this week (per schedule).
Global Nodes: Use the PDF protocol below.
Objective
Induce a reporter in a cell‑free transcription‑translation (TX‑TL) system and quantify expression changes between treatments.
Concepts You’ll Learn
- TX‑TL reaction components and setup
- Induction strategies and controls
- Fluorescence quantification & fold‑change analysis
Protocol
- Global Labs Protocol (PDF) — Cell_Free_System_Laboratory_(Global_Labs).pdf
Post‑Lab Questions (Mandatory)
- Using your lab images (or provided images), compute fluorescence fold‑change between treatments. Discuss LacI function in the context of the circuit used.
- What would you expect if the same induction were performed in E. coli BL21(DE3)?