Homework — Imaging and Measurement

Info

Homework this week is based on data generated at Waters Immerse Cambridge. You’ll characterize eGFP structure (intact mass; peptide map) using LC‑MS. Data will be provided online for remote students.

Final Project Homework

Warning
Mandatory for MIT/Harvard; optional for Committed Listeners (edited Apr 23).
  • Identify at least one aspect of your project you will measure (e.g., protein mass/sequence, biomarker presence/quantity).
  • Describe all elements you will measure and how you will perform these measurements.
  • Specify the technologies you will use (e.g., gel electrophoresis, DNA sequencing, mass spectrometry).

Waters Homework

Tip
Part 1 & 2 mandatory for Committed Listeners and MIT/Harvard students; Part 3 optional.
  1. Molecular weight — intact protein measurement.
  2. Primary amino acid sequence — peptide map.
  3. (Optional) Protein structure/shape — native vs denatured protein measurement.