Lab — Imaging & Measurement
Tip
This week’s practical focuses on LC‑MS workflows for eGFP characterization (intact mass and peptide mapping). On‑site work takes place at Waters Immerse Cambridge; remote students will use the shared dataset.
Objective
Connect measurement to design by acquiring and interpreting LC‑MS data.
Concepts You’ll Learn
- Sample prep for intact mass vs peptide mapping
- Fundamentals of LC‑MS instrumentation & readouts
- Native vs denatured protein measurements
- Basic analysis and documentation
