https://pages.htgaa.org/2026a/abhinav-rajendran/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Phusion DNA Polymerase: high-fidelity polymerase with 3’→5’ proofreading activity, so it corrects errors during extension. Much lower error rate than standard Taq. dNTPs (dATP, dTTP, dCTP, dGTP): the nucleotide building blocks the polymerase adds to the growing strand. MgCl₂: magnesium ions are an essential cofactor for polymerase function. Concentration affects stringency. Reaction buffer: maintains optimal pH and salt conditions across the three PCR temperature steps. The user adds template DNA and two primers (forward and reverse) to complete the reaction.Hugoen