Homework
Weekly homework submissions:
Week 1 HW: Principles and Practices
View Image Credits Image courtesy of Vincent Muir Q1. Describe a biological engineering application or tool you want to develop and why: Concept: Bio-Circuit for CO₂ Sensing and Reduction
Week 2 Homework: DNA Read, Write & Edit
3.1. Choose your protein Question: In recitation, we discussed that you will pick a protein for your homework that you find interesting. Which protein have you chosen and why? Using one of the tools described in recitation (NCBI, UniProt, google), obtain the protein sequence for the protein you chose. The protein I have chosen is Rubisco, the protein responsible for fixing carbon dioxide during photosynthesis. I have chosen this protein because one of my final project ideas was to create a system that is more efficient at fixing carbon.
Week 3: Automation Research & Project Proposal
- Published Automation Research Question: Find and describe a published paper that utilizes the Opentrons or an automation tool to achieve novel biological applications. The Paper Title: An automated cell-free platform for the rapid characterization of genetic regulators Journal: Scientific Reports (2024) Source: https://www.nature.com/articles/s41598-024-52642-x Description This research utilizes the Opentrons OT-2 to automate the setup of cell-free protein synthesis (CFPS) reactions. The authors focused on characterizing genetic parts like promoters and riboswitches. By using automation, they were able to test over 1,000 different conditions in a fraction of the time it would take a human, with much higher reproducibility across plates.