https://pages.htgaa.org/2026a/ade-larsen/homework/week-09-hw-cell-free-systems/index.htmlPart 1: General Homework Questions 1. Advantages of Cell-Free Synthesis Cell-free protein synthesis (CFPS) offers an “open” architecture, allowing for direct control over the chemical environment. Unlike in vivo methods, CFPS is not limited by cell toxicity or metabolic competition. Toxic Protein Production: CFPS can produce proteins that would otherwise kill a living host cell. High-Throughput Screening: It allows for the rapid testing of genetic libraries without the need for time-consuming cloning and cell culture. 2. Main Components Cell Extract: The catalytic machinery (ribosomes, polymerases). DNA Template: The genetic instructions. NTPs & Amino Acids: The energy and building blocks. Energy Regeneration: Essential for recycling ATP to sustain translation. 3. Energy Provision Energy regeneration is critical because ATP is consumed rapidly and phosphate byproducts inhibit the reaction. The Creatine Phosphate/Creatine Kinase system is often used to maintain an ATP supply by transferring phosphate groups to ADP.Hugoen