https://pages.htgaa.org/2026a/ade-larsen/labs/week-06-lab-gibson-assembly/index.htmlDay 1: Preparation of DNA Fragments Background In this two-day lab, we modified the color-generating chromophore of the purple Acropora millepora chromoprotein (amilCP) to create a variety of orange, pink, and blue mutants. We performed two sets of Polymerase Chain Reactions (PCR) to prepare for a Gibson Assembly. The insert PCR region spans the 24 base pairs before the chromophore to just beyond the gene transcription terminator. The forward primer includes an intentional mismatch for site-directed mutagenesis of the mUAV DNA plasmid. These mutants were then expressed in chemically competent E. coli cells.Hugoen