https://pages.htgaa.org/2026a/aditya-retnanto/homework/week-06-pcr-gibson-assembly/index.htmlPart A: Pre-Lab Protocol Questions 1. Phusion High-Fidelity PCR Master Mix According to the NEB product page, the Phusion master mix is built around two key features of the Phusion polymerase itself: A Pyrococcus-like polymerase core with a 3’→5’ proofreading exonuclease — catches and corrects misincorporated bases in real time, giving ~50x lower error rate than Taq. An Sso7d processivity domain fused to the polymerase — a DNA-binding clamp that keeps the enzyme on the template, increasing speed and fidelity together. The master mix also includes dNTPs, MgCl₂ (essential cofactor for polymerase activity), and a reaction buffer optimized for Phusion.Hugoen