https://pages.htgaa.org/2026a/aditya-retnanto/homework/week-10-mass-spec-final-project/index.htmlFinal Project: Measurements Plan What to Measure The chimeric casein project has three distinct questions that each need a different measurement approach: Stage 1 — Did the protein express? After inducing E. coli with IPTG and lysing the cells, I need to check whether the chimeric protein actually appeared. The primary tool for this is SDS-PAGE (gel electrophoresis for proteins) — the same ladder-based approach used for DNA, but with SDS added to unfold proteins and give them uniform charge so separation is purely by size. Running the pre- and post-induction lysate side by side, I’d look for a new band appearing at ~50 kDa (the expected molecular weight of the chimera). A Western blot using an anti-His antibody would then confirm the band is specifically my His-tagged chimera and not a coincidental band from the host cell.Hugoen