Week 3 HW: LAB AUTOMATION

How cool it is to desgin the logo of SAB at AUIB? the python scripit is uplouded

for the questions? Q1: a paper that utilizes Openrons for biological purposes:? I’ve came across this paper Titled: Automation of protein crystallization scale-up via Opentrons-2 (2025)

In Summary: it Uses an Opentrons OT‑2 robot to automate protein crystallization experiments, which are normally manual and laborious. The robot handles precise reagent dispensing, gradient formulation, and 96-well plate setup, increasing throughput and reproducibility. This is rare because low-cost robots are rarely used in structural biology workflows.

Q2:. With my final project including Adding plasmid to E. coli and culturing them. An automation Plan can be the following: Use Opentrons OT‑2 to pipette prepared plasmids into E. coli wells. Mix gently and adding growth medium. Before finally Incubating and grow colonies.

The Python Example: pipette.distribute(5, plasmid, ecoli_plate.rows()[0])

What else would be needed? Extras: 3D-printed tube holders, use Nebula for protocol tracking.