<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 6 — Genetic Circuits Part I: Assembly Technologies :: 2026a-alan-bravo</title><link>https://pages.htgaa.org/2026a/alan-bravo/homework/week-06-hw-genetic-circuits-part-i/index.html</link><description>Answer these questions about the protocol in this week’s lab:
What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? As stated in the thermofisher website, “Phusion DNA Polymerase, nucleotides, and optimized reaction buffer including MgCl2” The Phusion DNA Polymerase will be the one replicating the DNA at a really high fidelity The nucleotides will be ‘inserted’ into the replicated DNA strands And the reaction buffer (Including MgCl2, which is a co-factor needed for the enzyme), which is optimized, meaning, it has the favorable conditions for the enzyme such as pH.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/alan-bravo/homework/week-06-hw-genetic-circuits-part-i/index.xml" rel="self" type="application/rss+xml"/></channel></rss>