https://pages.htgaa.org/2026a/alayah-hines/labs/week-06-lab-gibson-assembly/index.htmlLab6 For lab this week we conducted a Gibson assembly to change the color of the purple Acropora millepora chromoprotein to a different mutant colors. I worked with Sean Murphy and Terry Luo, and we selected purple, orange, and light pink but ended up with 9 different petridishes to test our whole process, including: 4 x Purified DNA -> Gibson Assembly -> Transformation (but all mixed up and at much too low concentrations, there are four of these because one is all backbone) 3 x DNA straight from PCR -> Gibson Assembly -> Transformation (much higher concentrations but not purified) 1 x A mix of all of our purified DNA to see if we could get the concentration high enough and just see what happened 1 x A test with just the template plasmid A lot went wrong, so we really tried to troubleshoot.Hugoen