Week 7 Lab: Neuromorphic Circuits

Lab7

This week we designed our own intracellular artificial neural network using plasmids from the Ron Weiss lab and human embryonic kidney cells. I worked with Sean Murphy and Terry Luo and together we designed a comet. The heatmap had a high prediction value in a square region in the bottom left corner, which then narrowed down and then expanded like the tail of a comet.

Here was our spreadsheet, we used Csy4 as X1, Csy4_rec_CasE as X2, CasE_rec_mNeonGreen as Bias, PgU as X2, and PgU_rec_CasE as X1. We expected something like the bandpass example and used concentrations to make it fall off at higher concentrations of X1 and X2.

Here is its predicted associated heatmap

Here’s an image of the Opentrons building it in the Weiss lab

Finally, here’s how it turned out, not quite like the prediction and not quite like the bandpass we were building off of.