Homework
Weekly homework submissions:
Week 1 HW: Principles and Practices
Glowing Leather as a pilot for decentralized biomanufacturing of goods Bacterially produced leather is currently in the stage of being scaled up in centralized biomanufacturing plants around the world utilizing waste feedstocks from agricultural sources. Smaller scale experiments utilize kombucha SCOBYs to produce bacterial leather however they face variability in leather quality due to different growing conditions, feedstock, and SCOBY relationships in regards to each individual member’s reaction to the feedstock/growing conditions. The genetic drift that the SCOBY undergoes specifically the K. xylinus also makes it unreliable for consistent bacterial leather production. I am interested in exploring the development of an open source decentralized protocol to make growing bioleather easier, more climate resilient than growing cows, and more equitable than current centralized operations.
Week 2 HW: DNA Read, Write, & Edit
Week 2 Homework Part 1: Benchling & In-silico Gel Art MIT/Harvard students Required Committed Listeners Required See this week’s lab protocol “Gel Art: Restriction Digests and Gel Electrophoresis” for details. Overview: Make a free account at benchling.com Import the Lambda DNA Simulate Restriction Enzyme Digestion with the following enzymes: EcoRI HindIII BamHI KpnI EcoRV SacI SalI Create a pattern/image in the style of Paul Vanouse’s Latent Figure Protocol artworks You might find Ronan’s website a helpful tool for quickly iterating on designs! Response I was able to import all the restriction enzymes into benchling,
Homework Assignment: Python Script for Opentrons Artwork — DUE BY YOUR LAB TIME! Assignees for this section MIT/Harvard students Required Committed Listeners Required Your task this week is to Create a Python file to run on an Opentrons liquid handling robot.