LOCUS       BcsA _ Cellulose in pTwist Amp High Copy                4693 bp DNA     circular   20-FEB-2026
DEFINITION                               BcsA _ Cellulose in pTwist Amp High Copy
COMMENT                                  Resistance markers: Ampicillin

COMMENT                                  This file was created using tools provided by Twist Bioscience
FEATURES                                 Location/Qualifiers
     source                              1..4693
                                         /organism="BcsA _ Cellulose in pTwist Amp High Copy"
     misc_feature_                       1..2472
                                         /label="insert"
     misc_feature_                       2320..2336
                                         /label="6x_His_tag"
     misc_feature_                       2355..2398
                                         /label="rrnB_T1"
     misc_feature_                       2439..2458
                                         /label="stem_loop_early_T7"
     misc_feature_                       2473..4693
                                         /label="vector_backbone-2472-4693"
     misc_feature_                       2529..2545
                                         /label="m13_reverse"
     misc_feature_                       2717..3305
                                         /label="colE1_high_copy"
     misc_feature_                       3422..3446
                                         /label="attB1"
     misc_feature_                       3546..4406
                                         /label="ampR"
     misc_feature_                       4407..4511
                                         /label="AmpR_promoter"
ORIGIN
        1 TTTACGGCTA GCTCAGTCCT AGGTATAGTG CTAGCCATTA AAGAGGAGAA AGGTACCATG
       61 AGTGAGGTAC AATCACCAGT ACCAGCCGAG TCACGTTTGG ACAGATTCAG TAATAAGATT
      121 TTGTCTTTAC GCGGAGCTAA TTATATCGTT GGAGCGTTAG GATTATGTGC CTTAATTGCG
      181 GCCACGACGG TCACGCTCTC AATCAACGAG CAATTGATAG TGGCATTGGT TTGTGTCTTA
      241 GTGTTCTTCA TAGTCGGTCG TGGTAAGTCA AGAAGAACAC AAATCTTCCT TGAGGTACTT
      301 TCAGCCTTAG TTTCCCTTAG ATACTTAACA TGGCGACTTA CAGAGACATT AGACTTCGAC
      361 ACTTGGATCC AAGGTGGACT CGGAGTCACA TTGTTAATGG CCGAGTTGTA CGCTCTCTAC
      421 ATGTTATTCT TATCATATTT TCAAACAATC CAACCCCTTC ATCGGGCTCC ACTCCCCCTT
      481 CCCGACAACG TAGACGACTG GCCAACGGTT GACATATTCA TCCCTACATA CGACGAGCAA
      541 TTGTCTATCG TGCGCCTTAC GGTTTTGGGA GCCTTAGGTA TAGACTGGCC ACCTGACAAA
      601 GTTAACGTTT ATATATTGGA CGACGGTGTT CGCCCAGAGT TCGAGCAGTT TGCCAAGGAC
      661 TGTGGCGCGT TATACATAGG ACGGGTTGAC TCGTCCCATG CTAAGGCAGG AAATCTCAAC
      721 CATGCGATAA AGCGCACAAG TGGTGACTAT ATATTAATCC TTGACTGTGA CCATATACCC
      781 ACACGGGCGT TCTTACAAAT AGCCATGGGT TGGATGGTCG CTGATCGTAA GATAGCCCTT
      841 ATGCAAACGC CACACCACTT CTATTCACCT GACCCATTCC AGCGAAATTT AGCCGTTGGT
      901 TACAGAACAC CACCCGAGGG TAATCTTTTC TACGGCGTTA TTCAAGACGG GAATGACTTC
      961 TGGGACGCGA CTTTCTTCTG TGGTTCATGT GCAATATTGC GCCGTGAGGC AATAGAGTCA
     1021 ATAGGTGGGT TTGCAGTTGA AACGGTTACA GAGGACGCCC ACACAGCATT ACGTATGCAA
     1081 CGGCGAGGTT GGTCCACGGC ATACTTACGT ATCCCCGTAG CTTCTGGGTT AGCAACGGAG
     1141 CGCCTCACAA CACACATAGG TCAACGGATG CGGTGGGCCC GGGGTATGAT ACAAATATTC
     1201 CGAGTTGACA ATCCAATGTT GGGGGGGGGT CTTAAGTTAG GTCAACGTTT GTGCTATCTT
     1261 AGTGCAATGA CGTCATTCTT CTTTGCAATA CCACGTGTCA TCTTCTTGGC CTCACCATTA
     1321 GCCTTCCTCT TCTTCGGTCA AAATATAATT GCAGCATCTC CTTTAGCCGT TCTTGCGTAT
     1381 GCTATACCTC ACATGTTCCA TTCAATCGCT ACCGCAGCCA AGGTCAATAA GGGGTGGCGT
     1441 TACAGTTTCT GGTCCGAGGT CTATGAGACT ACAATGGCCC TTTTCCTTGT ACGCGTAACC
     1501 ATCATTACTT TAATGTTCCC AAGTAAGGGA AAGTTCAATG TCACCGAGAA GGGCGGTGTT
     1561 TTAGAGGAAG AGGAGTTCGA CCTCGGAGCC ACGTACCCAA ACATCATATT CGCTGGAATC
     1621 ATGACATTAG GTTTATTGAT AGGCTTGTTC GAGCTTACGT TTCATTTCAA CCAACTCGCC
     1681 GGTATCGCTA AGCGTGCATA CTTATTGAAC TGTATATGGG CTATGATCAG TTTAATCATA
     1741 CTTTTGGCAG CCATCGCTGT CGGTAGAGAG ACAAAGCAAG TACGTTACAA CCACCGAGTC
     1801 GAGGCACATA TCCCCGTTAC AGTCTACGAG GCTCCTGTCG CAGGTCAACC AAACACGTAC
     1861 CACAATGCCA CACCAGGGAT GACGCAGGAC GTATCGATGG GCGGTGTCGC TGTACATATG
     1921 CCATGGCCTG ATGTGAGTAC TGGGCCTGTC AAGACGCGAA TCCATGCCGT CTTAGACGGT
     1981 GAGGAGATCG ACATCCCAGC AACGATGTTA AGATGTAAGA ATGGTAAGGC CGTCTTTACT
     2041 TGGGACAATA ATGACTTAGA CACAGAGAGA GACATTGTAC GGTTCGTCTT TGGACGTGCG
     2101 GACGCCTGGC TTCAATGGAA TAACTATGAG GACGATCGGC CACTTCGCTC CTTGTGGTCG
     2161 TTGCTTCTTT CAATCAAGGC GCTCTTCCGT AAGAAGGGAA AGATGATGGC TAACAGTCGC
     2221 CCCAAGCGAA AGCCTTTGGC CTTACCTGTA GAGCGACGAG AGCCTACTAC AATCCAGTCA
     2281 GGCCAAACAC AAGAGGGAAA GATCTCACGA GCCGCTAGTC ACCATCACCA TCACCACCAT
     2341 TGACCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT
     2401 GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT
     2461 TCTGCGTTTA TAAGGCTAGG TGGAGGCTCA GTGATGATAA GTCTGCGATG GTGGATGCAT
     2521 GTGTCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCAGAGG GCACAATCCT
     2581 ATTCCGCGCT ATCCGACAAT CTCCAAGACA TTAGGTGGAG TTCAGTTCGG CGTATGGCAT
     2641 ATGTCGCTGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
     2701 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
     2761 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     2821 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
     2881 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
     2941 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
     3001 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
     3061 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
     3121 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC
     3181 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
     3241 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
     3301 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCTATTCAAC AAAGCCGCCG
     3361 TCCCGTCAAG TCAGCGTAAA TGGGTAGGGG GCTTCAAATC GTCCTCGTGA TACCAATTCG
     3421 GAGCCTGCTT TTTTGTACAA ACTTGTTGAT AATGGCAATT CAAGGATCTT CACCTAGATC
     3481 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     3541 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     3601 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     3661 GGCCCCAGTG CTGCAATGAT ACCGCGAGAG CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     3721 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     3781 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     3841 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     3901 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     3961 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     4021 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     4081 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     4141 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     4201 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     4261 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     4321 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     4381 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     4441 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     4501 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCAGATACCT GAAACAAAAC CCATCGTACG
     4561 GCCAAGGAAG TCTCCAATAA CTGTGATCCA CCACAAGCGC CAGGGTTTTC CCAGTCACGA
     4621 CGTTGTAAAA CGACGGCCAG TCATGCATAA TCCGCACGCA TCTGGAATAA GGAAGTGCCA
     4681 TTCCGCCTGA CCT
//