LOCUS       const_sfGFP_his-tag in pTwist Amp High Copy                3145 bp DNA     circular   16-APR-2026
DEFINITION                               const_sfGFP_his-tag in pTwist Amp High Copy
COMMENT                                  Resistance markers: Ampicillin

COMMENT                                  This file was created using tools provided by Twist Bioscience
FEATURES                                 Location/Qualifiers
     source                              1..3145
                                         /organism="const_sfGFP_his-tag in pTwist Amp High Copy"
     misc_feature_                       1..924
                                         /label="insert"
     misc_feature_                       61..771
                                         /label="GFP_ORF"
     misc_feature_                       807..850
                                         /label="rrnB_T1"
     misc_feature_                       891..910
                                         /label="stem_loop_early_T7"
     misc_feature_                       925..3145
                                         /label="vector_backbone-924-3145"
     misc_feature_                       981..997
                                         /label="m13_reverse"
     misc_feature_                       1169..1757
                                         /label="colE1_high_copy"
     misc_feature_                       1874..1898
                                         /label="attB1"
     misc_feature_                       1998..2858
                                         /label="ampR"
     misc_feature_                       2859..2963
                                         /label="AmpR_promoter"
ORIGIN
        1 TTTACGGCTA GCTCAGTCCT AGGTATAGTG CTAGCCATTA AAGAGGAGAA AGGTACCATG
       61 AGCAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT
      121 GTTAATGGGC ACAAATTTTC TGTCCGTGGA GAGGGTGAAG GTGATGCTAC AAACGGAAAA
      181 CTCACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCGTGGCC AACACTTGTC
      241 ACTACTCTGA CCTATGGTGT TCAATGCTTT TCCCGTTATC CGGATCACAT GAAACGGCAT
      301 GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG AACGCACTAT ATCTTTCAAA
      361 GATGACGGGA CCTACAAGAC GCGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT
      421 CGTATCGAGT TAAAGGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAACTC
      481 GAGTACAACT TTAACTCACA CAATGTATAC ATCACGGCAG ACAAACAAAA GAATGGAATC
      541 AAAGCTAACT TCAAAATTCG CCACAACGTT GAAGATGGTT CCGTTCAACT AGCAGACCAT
      601 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG
      661 TCGACACAAT CTGTCCTTTC GAAAGATCCC AACGAAAAGC GTGACCACAT GGTCCTTCTT
      721 GAGTTTGTAA CTGCTGCTGG GATTACACAT GGCATGGATG AGCTCTACAA ACATCACCAT
      781 CACCATCATC ACTAACCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT
      841 TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT
      901 CGGGTGGGCC TTTCTGCGTT TATAAGGCTA GGTGGAGGCT CAGTGATGAT AAGTCTGCGA
      961 TGGTGGATGC ATGTGTCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCAGA
     1021 GGGCACAATC CTATTCCGCG CTATCCGACA ATCTCCAAGA CATTAGGTGG AGTTCAGTTC
     1081 GGCGTATGGC ATATGTCGCT GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
     1141 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
     1201 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
     1261 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
     1321 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
     1381 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
     1441 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
     1501 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
     1561 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG
     1621 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
     1681 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
     1741 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCTATTCA
     1801 ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA AATGGGTAGG GGGCTTCAAA TCGTCCTCGT
     1861 GATACCAATT CGGAGCCTGC TTTTTTGTAC AAACTTGTTG ATAATGGCAA TTCAAGGATC
     1921 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     1981 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     2041 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2101 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AGCCACGCTC ACCGGCTCCA
     2161 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2221 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2281 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2341 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2401 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     2461 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     2521 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     2581 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     2641 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     2701 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2761 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     2821 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     2881 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     2941 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAGATAC CTGAAACAAA
     3001 ACCCATCGTA CGGCCAAGGA AGTCTCCAAT AACTGTGATC CACCACAAGC GCCAGGGTTT
     3061 TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTCATGCAT AATCCGCACG CATCTGGAAT
     3121 AAGGAAGTGC CATTCCGCCT GACCT
//