https://pages.htgaa.org/2026a/alexandra-burnusuz/homework/week-06-hw-gc-p1/index.htmlDNA Assembly Components in Phusion High-Fidelity PCR Master Mix and their purpose Phusion High-Fidelity PCR Master Mix typically contains a high-fidelity DNA polymerase (such as Phusion polymerase), dNTPs, Mg²⁺ (often as MgCl₂), and a reaction buffer. The high-fidelity polymerase has proofreading (3’→5’ exonuclease) activity, which reduces DNA replication errors.Hugoen