Part 1: Opentrons artwork

My attempt

Part 2: Use of automation tools

Find and describe a published paper that utilizes the Opentrons or an automation tool to achieve novel biological applications

Write a description about what you intend to do with automation tools for your final project. You may include example pseudocode, Python scripts, 3D printed holders, a plan for how to use Ginkgo Nebula, and more. You may reference this week’s recitation slide deck for lab automation details.

Well, of the three projects I’ve proposed, I’ll start with the second one, “Nostoc’s smart gummies”.

The objective here is to Characterize the sensitivity of heavy metal biosensors in Nostoc colonies and standardize the globular shape for the production of “gummies”

Cloud Lab Workflow (Ginkgo Nebula)

Echo: Precise gradient transfer of heavy metal solutions (lead and arsenic) at parts-per-billion (ppb) concentrations to Nostoc culture plates.

Multiflo: Dispensing of culture media optimized for cyanobacteria growth (BG-11).

PHERAstar: Measurement of the color intensity (absorbance) of reporter chromoproteins to establish the biosensor calibration curve.