https://pages.htgaa.org/2026a/alve-lagercrantz/homework/week-06-hw-genetic-circuits-part-i/index.htmlAssignment: DNA Assembly 1. What are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? The Phusion High-Fidelity PCR Master Mix contains at least three key components: Phusion DNA polymerase, deoxynucleotides (dNTPs), and an optimized reaction buffer that includes MgCl₂. The polymerase is the enzyme that synthesizes new DNA strands during PCR, the dNTPs are the nucleotide building blocks incorporated into the new DNA, and the buffer/MgCl₂ provide the chemical environment and cofactor needed for efficient polymerase activity. According to the website of (New England)[https://www.neb.com/en/products/m0531-phusion-high-fidelity-pcr-master-mix-with-hf-buffer?srsltid=AfmBOorWPUiBMtKsQJJH0VLGPzLYHtMYELtt0wf7AQB0YZYF4nrTfFsz] the main benefit of rgw Master mix is high fidelity (50X comparing to Taq) and fast extension times.Hugoen