https://pages.htgaa.org/2026a/alya-alobaidi/homework/week-06-hw-genetic-circuits-part-i/index.htmlDNA Assembly Phusion High-Fidelity PCR Master Mix contains DNA polymerase, dNTPs, buffer, and Mg²⁺. The polymerase has proofreading activity (3′→5′ exonuclease), which reduces errors during DNA amplification. The dNTPs act as building blocks, while the buffer and Mg²⁺ maintain optimal conditions for enzyme activity. This is important in cloning because even small mutations can affect protein function. https://www.neb.com/en/products/e0553-phusion-high-fidelity-pcr-kit?srsltid=AfmBOooI-JWWTJ01XuZL3foWSnvq5kqQol7r8q61xRo95a6S7amAGeiHHugoen