Design of a Glucocorticoid Receptor (GR-LBD)-Based Biosensor for Cortisol Detection Section1:Abstract Cortisol is one of the main hormones we look at when studying stress, circadian rhythm, and endocrine function, and it can be measured easily from saliva. It plays an important role in regulating multiple systems in the body, including metabolism, immune response, and cardiovascular function, which makes accurate measurement of cortisol important for understanding physiological stress. The problem is that most current detection systems rely on antibodies, which are not always stable, can be expensive, and do not perform well in complex environments like saliva. On top of that, cortisol is very similar in structure to cortisone, which makes it difficult to distinguish between the two and leads to noise and cross-reactivity in many biosensors.
