https://pages.htgaa.org/2026a/ana-gomez/homework/week-09-hw-cell-free-systems/index.htmlWeek 9: Cell-Free systems! Part A: General and Lecturer-Specific Questions General questions: Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. Cell-free protein synthesis (CFPS) offers important advantages over traditional in vivo expression because it provides a more open, flexible, and controllable reaction environment. Since there is no living cell to maintain, the researcher can directly adjust variables such as ionic strength, pH, redox conditions, DNA template concentration, cofactors, chaperones, detergents, lipids, or energy substrates without worrying about cell viability. CFPS is also typically faster, allowing protein production in hours rather than requiring cell growth, transformation, and induction steps over longer periods. In addition, it facilitates rapid prototyping of constructs and reaction conditions (Garenne et al., 2021; Jewett et al., 2008).Hugoen