Labs
Lab writeups:
Introduction to Pipetting and Dilutions 1. Concentration of the 5 M stock solution in g/mL Given: Stock concentration = 5 M Molecular weight = 532 g/mol Calculation: Answer The concentration of the stock solution is 2.66 g/mL.
The documentation of the virtual gel is in: Week 2: Dna-read-write-and-edit Assignment Please Check!
Week 3 Lab: Opentrons Art / Automation
The documentation of the Opetrons and Lab Automation is in: Week 3 HW: Lab Automation Please Check!
Week 6 — Genetic Circuits Part I Protocol: DNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix, and what is their purpose? The Phusion High-Fidelity PCR Master Mix contains several components required for efficient and accurate DNA amplification. The key components are: Phusion DNA Polymerase: A high-fidelity thermostable polymerase derived from Pyrococcus. It has proofreading (3’→5’ exonuclease) activity, which reduces the error rate during DNA synthesis
The documentation of the Cell-Free Systems lab is in: week-09-hw-cell-free-systems in “General Questions” Please Check!
Week 12 Lab: Bioproduction of Beta-Carotene and Lycopene
Bioproduction of Beta-Carotene and Lycopene 1. Enzymes of the carotene pathway CrtE, CrtB, CrtI, and CrtY are key enzymes involved in carotenoid biosynthesis. CrtE participates in geranylgeranyl pyrophosphate synthesis, CrtB catalyzes phytoene formation, CrtI mediates phytoene desaturation, and CrtY converts lycopene into beta-carotene. Although these enzymes are essential for carotenoid production, recent metabolic engineering studies suggest that carotenoid-specific enzymes do not solely limit lycopene yield. Instead, precursor availability, metabolic flux through the MEP pathway, cofactor balance, and overall cellular metabolism strongly influence production efficiency. (Huang et al., 2025; Jing et al., 2021; Sandmann & Misawa, 1992)