Labs
Lab writeups:
Week 6 — Genetic Circuits Part I Protocol: DNA Assembly What are some components in the Phusion High-Fidelity PCR Master Mix, and what is their purpose? The Phusion High-Fidelity PCR Master Mix contains several components required for efficient and accurate DNA amplification. The key components are: Phusion DNA Polymerase: A high-fidelity thermostable polymerase derived from Pyrococcus. It has proofreading (3’→5’ exonuclease) activity, which reduces the error rate during DNA synthesis