Homework
Weekly homework submissions:
Week 1 HW: Principles and Practices
First, describe a biological engineering application or tool you want to develop and why. This could be inspired by an idea for your HTGAA class project and/or something for which you are already doing in your research, or something you are just curious about. Implementing alternative methods against Pathogen-Ralstonia Solanacearum Raza 4 disease in platain and banana crops with wild bacteria. The identification and extraction of metabolites from wild isolated strain with high efficiency in the inhibition of the symptoms would help to stablish new alternatives
Week 2 hw: DNA, Read, Write & edit
Part 1: Benchling & In-silico Gel Art 1.1 Simulation of the enzyme digestion with the following enzymes Next to the ladder (Life 1kb Plus) there are the digestion from the enzymes:
EcoRI - HindIII - BamHI - KpnI - EcoRV - SacI - SalI Search the genome “Escherichia phage Lambda” (for this I used NCBI) Download or export the complete genome as a genbank file Open Benchling and import new DNA/RNA sequence (import the .gb file and rename) Now we have a visualization of our genome. Next, go to the right side of the bar and click in digestion (the scisors symbol). Select the restriction enzimes required and run each one. Click in “run digest” and visualize the simulation. For each one, I recommend to save each process so you can consult the results anytime. Benchling Workspace: https://benchling.com/s/seq-okBuD9NENrRXJIgK3yvh?m=slm-CAeF79q6rB0gWIBLHfkl
Assignment 1: Python Script for Opentrons Artwork Task 1.1: Create a Python file to run on an Opentrons liquid handling robot. For this asignment I used the GUI for generate the design and the coordinates. Then, with the assistance of GeminiAI, the coordinates were integrated using iterations. The pattern was divided and inserted in blocks and compilated part-by-part, until I was able to simulate the complete design and check its correct execution. For this work I used a pixel art design of the character Usagi from Chiikawa. There are also two more designs that I made as practice exercises. Also there is the link of the Usagi code for reference (Assisted with GeminiAI)