https://pages.htgaa.org/2026a/anastasia-ntavou/homework/week-10/index.htmlGlobal Listener — Anastasia Ntavou | Athens, Greece Project: Mycelium Surfboard (Ganoderma lucidum engineering) Final Project Measurement Plan 1. SC16 Hydrophobin Expression Verification What: Presence and molecular weight of SC16 (~10 kDa) Method: SDS-PAGE + anti-His western blot Alternative: MALDI-TOF MS — confirms exact MW and detects truncations 2. Water Contact Angle (WCA) What: Surface hydrophobicity of engineered vs. wild-type mycelium Method: Sessile drop goniometry — 5µL drop on mycelium-coated glass Target: > 120° (engineered) vs. < 30° (wild-type) 3. Disulfide Bond Verification What: Correct SC16 folding (4 disulfide bonds essential) Method: Non-reducing SDS-PAGE + MS peptide mapping 4. Composite Mechanical Properties What: Flexural strength, compressive strength, modulus Method: ASTM D790 (3-point bend), ASTM D695 (compression) 5. Compostability What: Mass loss over 28 days Method: ASTM D5338 at 58°C / 60% humidity Target: > 90% mass loss Waters Mass Spectrometry Questions Part I — eGFP Molecular Weight Using ExPASy ProtParam on the provided sequence (239 aa with His-tag):Hugoen