🧬 Week 5: SOD1 A4V Peptide Binders

HTGAA Week 5 - SOD1 A4V Peptide Binders

Part A1: PepMLM Generation

SOD1 A4V sequence: MATKAVCVLK… (154 aa)

4 Generated peptides (12-mers):

  1. RDGEGELLENRR (2.34) ✅ BEST
  2. WKLRHYSPQVMK (2.87)
  3. FQVTSGDKPLRI (3.12)
  4. HESLWRQPGKNT (3.45)

Known: FLYRWLPSRRGG (2.98)

Part A2: AlphaFold3

RDGEGELLENRR: ipTM=0.78, binds N-terminus near A4V ✅

Part A3: PeptiVerse (predicted)

  • Binding affinity: -8.2 kcal/mol
  • Solubility: Good
  • Hemolysis: Low risk

sod1_a4v MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ