https://pages.htgaa.org/2026a/andrei-vasilan/homework/week-06-genetic-circuits-part-1/index.htmlWhat are some components in the Phusion High-Fidelity PCR Master Mix and what is their purpose? Components: Phusion DNA Polymerase (low error rate), nucleotides (A, T, C, G without U), reaction buffer (ensure maximum enzyme activity); What are some factors that determine primer annealing temperature during PCR? Each primer has a different annealing temperature determined by the environment and by the nucleotides that make up the primer There are two methods from this class that create linear fragments of DNA: PCR, and restriction enzyme digests. Compare and contrast these two methods, both in terms of protocol as well as when one may be preferable to use over the other. PCR makes strands by attaching primers with tails that hang freely. The result is a DNA sequence made of the original one + the tails of the primers that were added. Restriction enymes cut DNA and the resulted sequence part of the original sequence.Hugoen