The treatment of metastatic cancer remains a significant clinical challenge due to the dynamic and heterogeneous nature of the tumor microenvironment. Current therapeutic interventions often lack the requisite precision to adapt to these rapid physiological changes, frequently leading to sub-optimal outcomes and drug resistance. The broad objective of this project is to engineer a Bacterial Microcompartment (BMC) nanocage integrated with an Intracellular Artificial Neural Network (IANN) genetic circuit. This system is designed to autonomously sense multifaceted biochemical stimuli within a tumor and respond by secreting a calibrated dose of anti-cancer enzymes and immune-boosting proteins. This nanocage would remain dormant inside your body similar to a sentinel. It would be activated only when a special in encounters its activation protein that must be injected into your bloodstream from outside. Aim 1: Designing and testing the genetic circuit in a cell free system