Homework

Weekly homework submissions:

• Week 1 HW: Principles and Practices

  Class Assignments

• week-02-hw-dna-read-write-and-edit

  Part 0: Basics of Gel Electrophoresis I watched the lectures by George Church, Joe Jacobson, and Emily Leproust, as well as the recitation by Ice Kiattisewee on DNA Gel, restriction enzymes, Benchling, and Twist. Key takeaways from lectures: Gel electrophoresis works by taking advantage of the negative charge of the DNA molecule due to the phosphate group. DNA fragments being placed in the agarose gel (which is heated and then cooled) move according to their size under the effect of the electric field applied, separating based on their size. The pore size of the agarose gel is determined by the concentration of agarose - (with higher concentrations –> decreased pore size –> smaller fragments/finer fragments being separated easily. The ladder containing the weight of the DNA in kDa helps in estimating the size of the DNA based on its position in the gel. Ethidium bromide is used for staining resulting in the DNA to glow under the UV light.

• Week 3: Lab Automation & Opentrons Art

  Part 1: Opentrons Agar Art - Biohazard Symbol Design Concept I conceptualised the biohazard symbol, which I thought of creating using parametric algorithms. I used the assistance of Claude (Anthropic) to generate the python code which I ran on Google Colab, in the copy provided by HTGAA. The results are shown below.