Homework

Weekly homework submissions:

• Week 1 HW: Principles and Practices

  1.Biological engineering tool/application I am trying to develop a dyeing method for fabrics and surfaces by using Physarum Polycephalum, or the slime mould as an activator. The aim is to let the slime mould create one-of-one designs by growing on the surface, letting a level of unpredictabiity of growth control the outcome. Slime moulds are very good at creating pathways while expanding in search of optimum survival conditons. During this travel, they tend to leave behind residual pigment, usually yellow in colour. After drying it looks something like this. In this bioengineered application, physarum polycephalum expresses a pigment forming enzyme(tyrosinase/laccase-type oxidase) that catalyzes the oxidation of benign phenolic or cathechol precursors into reactive quinones that polymerize into and insoluble melanin-like pigment.

• Week 2 HW: DNA read-write-edit

  Part 1: Gel Electrophoresis Due to no access to equipment and space for gel electrophoresis I simulated the same to understand the process on https://www.labxchange.org/library/items/lb:LabXchange:9548bee3:lx_simulation:1?fullscreen=true Workflow Design plasmid DNA with protein of interest →Transform bacteria with plasmid DNA→Get many copies of plasmid DNA→introduction of plasmid DNA to cells