https://pages.htgaa.org/2026a/ariadna-abigail-ruiz-castro/homework/week-9--cell-free-systems/index.htmlHomework Part A: General and Lecturer-Specific Questions General homework questions Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. The primary advantage lies in the decoupling of the reaction from cellular metabolism. ​Flexibility: It allows the use of linear DNA, eliminates the need for transformation and host-specific codon optimization, and facilitates the expression of proteins that are toxic to the host. ​Control of variables: It is an “open” system. You can manipulate buffer composition (pH, ionic strength), add chaperones, modify the Mg2+/K+ ratio, or add specific redox agents for disulfide bond formation in real-time, without the limitations of cellular homeostasis. ​Use cases: ​Toxic proteins: Production of proteins that compromise host viability (e.g., antimicrobial peptides or nucleases). ​Non-canonical amino acid (ncAA) incorporation: Facilitates genetic code expansion via stop codon suppression without competition from endogenous tRNAs.Hugoen