https://pages.htgaa.org/2026a/asaf-balaga/labs/week-06/index.htmlThis week our lab was about the Gibson Assembly process. We edited an exisiting plasmid by fragmenting it and ‘stiching’ it back with a mutated fragment. Our goal was to introduce a mutation of the chromophore of amilCP - a purple chromoprotein originally from the coral Acropora millepora - to generate new color variants that would be expressed in E.Coli bacteria. We did so by using PCR primers that already had the color mutations incorporated, and assembled it into a plasmid containing the elements needed for replication and expression in bacteria. The plasmid was then transformed into E. coli so the cells grow and express the mutated protein.Hugoen