Week 2 HW: DNA Read Write and Edit
Table of Contents
- Part 0: Basics of Gel Electrophoresis
- Part 1: Benchling and In-silico Gel Art
- Part 2: Gel Art - Restriction Digests and Gel Electrophoresis
- Part 3: DNA Design Challenge
- Part 4: Prepare a Twist DNA Synthesis Order
- Part 5: DNA Read/Write/Edit
Part 0: Basics of Gel Electrophoresis
Watched lecture, recitation AND the bootcamp 🫡
Part 1: Benchling and In-silico Gel Art
In Benchling, follow the instructions as outlined in the guide video:
- Click “+” (create)
- DNA/RNA Sequence $\rightarrow$ New DNA/RNA sequence
- Name: “Lambda Sequence”
- Nucleotide type: DNA, Topology: Linear
- Bases: copy and paste the sequence of bases in
- Click the scissors tool to digest, and search for each of the seven enzymes. After each individual enzyme is selected click “Run digest” to add a new lane to your virtual digest
Part 2: Gel Art - Restriction Digests and Gel Electrophoresis
Can’t perform - no wet lab access.
Part 3: DNA Design Challenge
3.1. Choose your protein
I’ve chosen mECFP, it’s a fluorescent protein that produces a lovely cyan/blue colour. It was my favourite fluorescent protein I saw in my quantitative optical imaging/microscopy class.