HTGAA'26 — BEYZA BATIR — PROPOSAL IDEAS
Idea.4: CRYO-LUME
Spherified biosensors for aesthetic contaminant detection. Engineered E. coli in hydrophobic alginate spheres detect arsenic, glow via luxCDABE bioluminescence, and trigger ice nucleation via inaK protein in supercooled water.
Supercooled water chamber — click to drop extra spheres
Alginate sphere
Bioluminescence
Ice crystal
Arsenic zone
Parameters
50
15
2.0
100
-3
0
Spheres
0
Glowing
0
Frozen
—
Avg RLU
—
Nuc. temp
Experiment log
| # | As (ppb) | Spheres | Alginate | CaCl2 | Temp | Glow | Frozen | Avg RLU | Nuc. T |
|---|
Drop spheres to start collecting data.
96-well dose-response (Opentrons)
0 ppb500 ppb
RLU dose-response curve
RLU/OD600
Nuc. threshold