Homework

Weekly homework submissions:

• Week 2 HW: DNA ~~ Read, Write, and Edit

  Part 1: Benchling & In-silico Gel Art Make a free account at benchling.com Import the Lambda DNA. Simulate Restriction Enzyme Digestion with the following Enzymes: EcoRI HindIII BamHI KpnI EcoRV SacI SalI Create a pattern/image in the style of Paul Vanouse’s Latent Figure Protocol artworks. You might find Ronan’s website a helpful tool for quickly iterating on designs! [[lambaNEB.png]] 3: DNA Design Challenge 3.1. Choose your protein. In recitation, we discussed that you will pick a protein for your homework that you find interesting. Which protein have you chosen and why? Using one of the tools described in recitation (NCBI, UniProt, google), obtain the protein sequence for the protein you chose. Have chosen Channelrhodopsin-2 a transmembrane ion transporter whose open/close state is dependent upon UV light hitting it. This basically means that we have unprecedented control over supressing or inducing neuronal activation via ion flow into cell, leading to exzternal neuronal control with some pretty crazy behavioural studies discovering the neuronal circuitry regulating behaviours such as eating, satiety, agression, and even target tracking during hunting in mice and dogs.