Homework
Weekly homework submissions:
Week 1 HW: Principles and Practices
First, describe a biological engineering application or tool you want to develop and why. Engineered bacteriophage as a delivery vector or indicator. Factors such as selective host range, ability to integrate, infection of drug-resistant bacteria, reproducibility, and more would make them a versatile tool. Next, describe one or more governance/policy goals related to ensuring that this application or tool contributes to an “ethical” future, like ensuring non-malfeasance (preventing harm). Break big goals down into two or more specific sub-goals. (slightly edited from example framework)
Week 2 HW: DNA Read, Write, and Edit
Part 1: Benchling & In-silico Gel Art My node (W&M) is using one of our phages’(Kampy) DNA for the Gel Art DNA - https://phagesdb.org/phages/Kampy/ Our lab’s restriction enzymes: AfIII Age1 AluI ApaI BamHI-HF BbsI BsaI-HF BglI BstXI DpnI Eco0109I Eco47III EcoICRI EcoRI-HF EcoRV HindIII I-SceI KpnI NotI-HF PspAI PstI-HF SpaI-HF XbaI XhoI Part 2: Gel Art - Restriction Digests and Gel Electrophoresis Our design
Python Script for Opentrons Artwork Designs Using the GUI at opentrons-art.rcdonovan.com Using custom design tools (trying out stippling, different dot sizes) Python Files What the different dot design looks like after programming so far (still need to scale a bit)