https://pages.htgaa.org/2026a/dan-wright/homework/week-06-hw-genetic-circuits-part-i/index.htmlLab Protocol: PCR, Digestion, and Assembly Strategies1. Phusion High-Fidelity PCR Master MixPhusion is the standard for cloning due to its high speed and accuracy.ComponentPurposePhusion DNA PolymeraseA “Pyrococcus-like” enzyme fused to a dsDNA-binding domain. This provides extreme processivity and 3’→5’ exonuclease activity (proofreading) for high fidelity.dNTPsDeoxynucleotide triphosphates ($A, T, C, G$)—the raw building blocks for DNA synthesis.Phusion HF (or GC) BufferMaintains optimal pH and provides $MgCl_2$ cofactors essential for polymerase activity.Hot Start AdditivesReversible inhibitors that prevent non-specific amplification at room temperature.Hugoen