Transferring 2.4 from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from A1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 2.4 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 2.4 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 2.4 from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from B1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 2.4 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 2.4 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 2.4 from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from C1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 2.4 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 2.4 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 2.4 from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from D1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 2.4 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 2.4 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 3.0 from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from E1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 3.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 3.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 3.0 from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from F1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 3.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 3.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Pausing robot operation: Now, get your OM and P3000 and place in tuberack at the locations specified on the spreadsheet

Transferring 2.0592 from D4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Picking up tip from G1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 2.0592 uL from D4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 2.0592 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Blowing out at D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Dropping tip into Trash Bin on slot 12

Transferring 46.8 from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Picking up tip from A1 of Opentrons OT-2 96 Tip Rack 300 µL on slot 9

Aspirating 46.8 uL from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Mixing 3 times with a volume of 46.8 ul

Aspirating 46.8 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Aspirating 46.8 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Aspirating 46.8 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Blowing out at D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Dropping tip into Trash Bin on slot 12

Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from H1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 15.0 ul

Aspirating 15.0 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from A2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 15.0 ul

Aspirating 15.0 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from B2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 15.0 ul

Aspirating 15.0 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from C2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 15.0 ul

Aspirating 15.0 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 7.83 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from D2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 7.83 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 7.83 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 15.0 ul

Aspirating 15.0 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 7.83 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from E2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 7.83 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 7.83 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 15.0 ul

Aspirating 15.0 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 15.0 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Pausing robot operation: Now, get your L3000 and place in tuberack at the location specified on the spreadsheet

Transferring 2.0592 from D3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Picking up tip from F2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 2.0592 uL from D3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 2.0592 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Blowing out at D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Dropping tip into Trash Bin on slot 12

Transferring 46.8 from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Picking up tip from B1 of Opentrons OT-2 96 Tip Rack 300 µL on slot 9

Aspirating 46.8 uL from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Mixing 3 times with a volume of 46.8 ul

Aspirating 46.8 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Aspirating 46.8 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Aspirating 46.8 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec

Blowing out at D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6

Dropping tip into Trash Bin on slot 12

Picking up tip from C1 of Opentrons OT-2 96 Tip Rack 300 µL on slot 9

Picking up tip from G2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 6.264 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Transferring 7.83 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Aspirating 7.83 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 7.83 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Transferring 7.83 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Aspirating 7.83 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec

Dispensing 7.83 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Dropping tip into Trash Bin on slot 12

Transferring 8.664 from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from H2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 8.664 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 8.664 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 8.664 from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from A3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 8.664 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 8.664 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 8.664 from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from B3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 8.664 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 8.664 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 8.664 from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from C3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 8.664 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 8.664 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 10.83 from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from D3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 10.83 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 10.83 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Transferring 10.83 from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Picking up tip from E3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 10.83 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 10.83 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Mixing 3 times with a volume of 20.0 ul

Aspirating 20.0 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Aspirating 20.0 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 20.0 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Blowing out at C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4

Dropping tip into Trash Bin on slot 12

Pausing robot operation: Now, incubate the mixture for 10 mins and get your cells and place in the deck specified in the OT-2 protocol

Transferring 12.440000000000001 from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Picking up tip from F3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 12.440000000000001 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec

Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Dropping tip into Trash Bin on slot 12

Transferring 12.440000000000001 from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Picking up tip from G3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 12.440000000000001 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec

Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Dropping tip into Trash Bin on slot 12

Transferring 12.440000000000001 from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Picking up tip from H3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 12.440000000000001 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec

Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Dropping tip into Trash Bin on slot 12

Transferring 12.440000000000001 from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Picking up tip from A4 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 12.440000000000001 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec

Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Dropping tip into Trash Bin on slot 12

Transferring 15.55 from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Picking up tip from B4 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 15.55 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.55 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec

Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Dropping tip into Trash Bin on slot 12

Transferring 15.55 from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Picking up tip from C4 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8

Aspirating 15.55 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec

Dispensing 15.55 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec

Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2

Dropping tip into Trash Bin on slot 12

Stderr:

C:\Users\x\.opentrons\robot_settings.json not found. Loading defaults

Deck calibration not found.

C:\Users\x\.opentrons\deck_calibration.json not found. Loading defaults

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✓ Simulation completed successfully