<link>https://pages.htgaa.org/2026a/dan-wright/homework/week-07-hw-genetic-circuits-part-ii/neuromorphic-wizard/index.html</link><description><!DOCTYPE html> Neuromorphic Wizard Neuromorphic WizardBuildPredictGenerateAnalyzechevron_leftchevron_rightExperiment Designdone_allUpload File (.csv, .json, .py)375.0B / 100.00%add_boxhelp me design a genetic circuit using the attach... - help me design a genetic circuit using the attach....csv375.0B / 100.00%doneOT-2 Template:v3.9arrow_drop_downNo Rows To Show Circuit name Transfection group Contents Concentration (ng/uL) DNA wanted (ng) DNA source DNA destination L3K/OM MM destination Plate destination Diluted source CancerDetectorX1 (Input High)CasE50100A1.1B1.1C1.1A1.1CancerDetectorX2 (Input Low)PgU50100A2.1B2.1C2.1A1.1CancerDetectorX3 (Intermediary)Csy450100A3.1B3.1C3.1A1.1CancerDetectorX3 (Logic)CasE_rec_Csy450100A4.1B4.1C4.1A1.1CancerDetectorBias (Output A)Csy4_rec_mNeonGreen50125A5.1B5.1C5.1A1.1CancerDetectorBias (Output B)PgU_rec_mNeonGreen50125A6.1B6.1C6.1A1.1 to of Page of addAdd Rowdelete_sweepDelete AllDelete circuit:Select Circuitarrow_drop_downdelete_foreverDeleteExperiment LayoutCreate LayoutrefreshReset LayoutInput #1: 24-Tube Rack (OT-2 Slot 4)123456ACasEPgUCsy4CasE_...Csy4_...PgU_r...BCance...Cance...Cance...Cance...Cance...Cance...CCance...Cance...Cance...Cance...Cance...Cance...DInput #2: 24-Tube Rack (OT-2 Slot 5)123456ABCDInput #3: 24-Tube Rack (OT-2 Slot 6)123456ABCDH2OL3K/P...Reser...P3000Reser...Opti-...Output #1: 24-Well Plate (OT-2 Slot 2)123456ACance...BCDOutput #2: 24-Well Plate (OT-2 Slot 3)123456ABCDOpentrons SimulationSimulateSimulation OutputTransferring 2.4 from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from A1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 2.4 uL from A1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 2.4 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 2.4 from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from B1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 2.4 uL from A2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 2.4 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 2.4 from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from C1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 2.4 uL from A3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 2.4 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 2.4 from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from D1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 2.4 uL from A4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 2.4 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 3.0 from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from E1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 3.0 uL from A5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 3.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 3.0 from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from F1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 3.0 uL from A6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 3.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Pausing robot operation: Now, get your OM and P3000 and place in tuberack at the locations specified on the spreadsheetTransferring 2.0592 from D4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Picking up tip from G1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 2.0592 uL from D4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 2.0592 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Blowing out at D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Dropping tip into Trash Bin on slot 12Transferring 46.8 from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Picking up tip from A1 of Opentrons OT-2 96 Tip Rack 300 µL on slot 9 Aspirating 46.8 uL from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Mixing 3 times with a volume of 46.8 ul Aspirating 46.8 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Aspirating 46.8 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Aspirating 46.8 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Blowing out at D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Dropping tip into Trash Bin on slot 12Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from H1 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 15.0 ul Aspirating 15.0 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from A2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 15.0 ul Aspirating 15.0 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from B2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 15.0 ul Aspirating 15.0 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 6.264 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from C2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 6.264 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 15.0 ul Aspirating 15.0 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 7.83 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from D2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 7.83 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 7.83 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 15.0 ul Aspirating 15.0 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 7.83 from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from E2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 7.83 uL from D2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 7.83 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 15.0 ul Aspirating 15.0 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 15.0 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.0 uL into B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Pausing robot operation: Now, get your L3000 and place in tuberack at the location specified on the spreadsheetTransferring 2.0592 from D3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Picking up tip from F2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 2.0592 uL from D3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 2.0592 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Blowing out at D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Dropping tip into Trash Bin on slot 12Transferring 46.8 from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Picking up tip from B1 of Opentrons OT-2 96 Tip Rack 300 µL on slot 9 Aspirating 46.8 uL from D6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Mixing 3 times with a volume of 46.8 ul Aspirating 46.8 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Aspirating 46.8 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Aspirating 46.8 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Dispensing 46.8 uL into D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 250.0 uL/sec Blowing out at D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 Dropping tip into Trash Bin on slot 12Picking up tip from C1 of Opentrons OT-2 96 Tip Rack 300 µL on slot 9Picking up tip from G2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4Transferring 6.264 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Aspirating 6.264 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 6.264 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4Transferring 7.83 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Aspirating 7.83 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 7.83 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4Transferring 7.83 from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 to C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Aspirating 7.83 uL from D1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 6 at 20.0 uL/sec Dispensing 7.83 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4Dropping tip into Trash Bin on slot 12Dropping tip into Trash Bin on slot 12Transferring 8.664 from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from H2 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 8.664 uL from B1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 8.664 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 8.664 from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from A3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 8.664 uL from B2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 8.664 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 8.664 from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from B3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 8.664 uL from B3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 8.664 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 8.664 from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from C3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 8.664 uL from B4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 8.664 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 10.83 from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from D3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 10.83 uL from B5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 10.83 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Transferring 10.83 from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Picking up tip from E3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 10.83 uL from B6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 10.83 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Mixing 3 times with a volume of 20.0 ul Aspirating 20.0 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Aspirating 20.0 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 20.0 uL into C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Blowing out at C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 Dropping tip into Trash Bin on slot 12Pausing robot operation: Now, incubate the mixture for 10 mins and get your cells and place in the deck specified in the OT-2 protocolTransferring 12.440000000000001 from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Picking up tip from F3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 12.440000000000001 uL from C1 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Dropping tip into Trash Bin on slot 12Transferring 12.440000000000001 from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Picking up tip from G3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 12.440000000000001 uL from C2 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Dropping tip into Trash Bin on slot 12Transferring 12.440000000000001 from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Picking up tip from H3 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 12.440000000000001 uL from C3 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Dropping tip into Trash Bin on slot 12Transferring 12.440000000000001 from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Picking up tip from A4 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 12.440000000000001 uL from C4 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 12.440000000000001 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Dropping tip into Trash Bin on slot 12Transferring 15.55 from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Picking up tip from B4 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 15.55 uL from C5 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.55 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Dropping tip into Trash Bin on slot 12Transferring 15.55 from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 to A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Picking up tip from C4 of Opentrons OT-2 96 Tip Rack 20 µL on slot 8 Aspirating 15.55 uL from C6 of Opentrons 24 Tube Rack with Eppendorf 1.5 mL Safe-Lock Snapcap on slot 4 at 20.0 uL/sec Dispensing 15.55 uL into A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 at 20.0 uL/sec Blowing out at A1 of Corning 24 Well Plate 3.4 mL Flat on slot 2 Dropping tip into Trash Bin on slot 12Stderr:C:\Users\x\.opentrons\robot_settings.json not found. Loading defaultsDeck calibration not found.C:\Users\x\.opentrons\deck_calibration.json not found. Loading defaults============================================================✓ Simulation completed successfullyDownloadSelect DownloadAll Files (.zip)arrow_drop_downFilename (without extension)Download Connection lost. Trying to reconnect... Message too long The message is too large for WebSocket transmission.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/dan-wright/homework/week-07-hw-genetic-circuits-part-ii/neuromorphic-wizard/index.xml" rel="self" type="application/rss+xml"/></channel></rss>