https://pages.htgaa.org/2026a/daniel-tseng/homework/week-03-hw-lab-automation/index.htmlGUI design Opentron Python Script for Opentrons Artwork from opentrons import protocol_api from opentrons.protocol_api import SINGLE from opentrons.types import Point metadata = { 'protocolName': 'Multichannel Color Dispensing (Single Tip) - Dani', 'author': '', 'description': 'Aspirate colors column-wise with a single tip on an ' '8-channel pipette and dispense into virtual grid ' 'positions in a reservoir.', 'apiLevel': '2.20' } # --------------------------------------------------------------------------- # USER CONFIGURATION # --------------------------------------------------------------------------- VOLUME_PER_DROP = 2 DISPENSE_HEIGHT = 0 START_TIP = "E1" DROP_TIP_IN_TRASH = True # Usable volume per well (leave margin below 250 µL max) WELL_CAPACITY = 220 # µL # --------------------------------------------------------------------------- # COLOR MAP # IMPORTANT: use "source_wells" (plural), not "source_well" # --------------------------------------------------------------------------- mscarlet_i_points = [(-7.7, 38.5),(-5.5, 38.5),(-3.3, 38.5),(-1.1, 38.5),(1.1, 38.5),(3.3, 38.5),(5.5, 38.5),(7.7, 38.5),(-14.3, 36.3),(-12.1, 36.3),(-9.9, 36.3),(-7.7, 36.3),(-5.5, 36.3),(-3.3, 36.3),(-1.1, 36.3),(1.1, 36.3),(3.3, 36.3),(5.5, 36.3),(7.7, 36.3),(9.9, 36.3),(12.1, 36.3),(14.3, 36.3),(-20.9, 34.1),(-18.7, 34.1),(-16.5, 34.1),(-14.3, 34.1),(-12.1, 34.1),(-9.9, 34.1),(-7.7, 34.1),(-5.5, 34.1),(-3.3, 34.1),(-1.1, 34.1),(1.1, 34.1),(3.3, 34.1),(5.5, 34.1),(7.7, 34.1),(9.9, 34.1),(12.1, 34.1),(14.3, 34.1),(16.5, 34.1),(18.7, 34.1),(20.9, 34.1),(-23.1, 31.9),(-20.9, 31.9),(-18.7, 31.9),(-16.5, 31.9),(-14.3, 31.9),(-12.1, 31.9),(-9.9, 31.9),(-7.7, 31.9),(-5.5, 31.9),(-3.3, 31.9),(-1.1, 31.9),(1.1, 31.9),(3.3, 31.9),(5.5, 31.9),(7.7, 31.9),(9.9, 31.9),(12.1, 31.9),(14.3, 31.9),(16.5, 31.9),(18.7, 31.9),(20.9, 31.9),(23.1, 31.9),(-25.3, 29.7),(-23.1, 29.7),(-20.9, 29.7),(-18.7, 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-31.9),(5.5, -31.9),(7.7, -31.9),(9.9, -31.9),(12.1, -31.9),(14.3, -31.9),(16.5, -31.9),(18.7, -31.9),(20.9, -31.9),(23.1, -31.9),(-20.9, -34.1),(-18.7, -34.1),(-16.5, -34.1),(-14.3, -34.1),(-12.1, -34.1),(-9.9, -34.1),(-7.7, -34.1),(-5.5, -34.1),(-3.3, -34.1),(-1.1, -34.1),(1.1, -34.1),(3.3, -34.1),(5.5, -34.1),(7.7, -34.1),(9.9, -34.1),(12.1, -34.1),(14.3, -34.1),(16.5, -34.1),(18.7, -34.1),(20.9, -34.1),(-14.3, -36.3),(-12.1, -36.3),(-9.9, -36.3),(-7.7, -36.3),(-5.5, -36.3),(-3.3, -36.3),(-1.1, -36.3),(1.1, -36.3),(3.3, -36.3),(5.5, -36.3),(7.7, -36.3),(9.9, -36.3),(12.1, -36.3),(14.3, -36.3),(-7.7, -38.5),(-5.5, -38.5),(-3.3, -38.5),(-1.1, -38.5),(1.1, -38.5),(3.3, -38.5),(5.5, -38.5),(7.7, -38.5)] azurite_points = [(-5.4, 23.4),(-1.8, 23.4),(1.8, 23.4),(5.4, 23.4),(-9, 19.8),(-5.4, 19.8),(-1.8, 19.8),(1.8, 19.8),(5.4, 19.8),(9, 19.8),(12.6, 19.8),(12.6, 16.2),(12.6, 12.6),(-12.6, 9),(-1.8, 9),(1.8, 9),(5.4, 9),(12.6, 9),(-12.6, 5.4),(12.6, 5.4),(-12.6, 1.8),(12.6, 1.8),(-12.6, -1.8),(-9, -1.8),(-5.4, -1.8),(-1.8, -1.8),(1.8, -1.8),(5.4, -1.8),(-12.6, -5.4),(-5.4, -12.6)] mko2_points = [(9, 23.4),(-12.6, 16.2),(12.6, -1.8),(-9, -5.4),(1.8, -5.4),(16.2, -9),(-12.6, -12.6),(-9, -16.2),(-5.4, -16.2),(-1.8, -16.2),(-12.6, -23.4),(-9, -23.4),(-5.4, -23.4),(-1.8, -23.4),(1.8, -23.4),(5.4, -23.4),(9, -23.4),(-5.4, -30.6),(-1.8, -30.6),(1.8, -30.6),(5.4, -30.6),(9, -30.6),(12.6, -30.6),(16.2, -34.2)] sfgfp_points = [] COLOR_MAP = [ {'name': 'Red', 'source_wells': ['A1','B1','C1','D1','E1','F1','G1','H1'], 'points': mscarlet_i_points} ] # --------------------------------------------------------------------------- # RESERVOIR BOUNDS # --------------------------------------------------------------------------- RESERVOIR_X_MM = 107.0 RESERVOIR_Y_MM = 67.0 ALLOW_OUT_OF_BOUNDS = False # --------------------------------------------------------------------------- # PROTOCOL # --------------------------------------------------------------------------- def run(protocol: protocol_api.ProtocolContext): # --- Labware --- tip_rack = protocol.load_labware('opentrons_96_tiprack_20ul', 6) plate = protocol.load_labware('scienfocus_96_wellplate_250ul', 1) reservoir = protocol.load_labware('thermo_1_reservoir_90000ul', 2) # --- Pipette --- pipette = protocol.load_instrument('p20_multi_gen2', 'left', tip_racks=[tip_rack]) pipette.configure_nozzle_layout(style=SINGLE, start="H1") # --- Reference locations --- reservoir_well = reservoir['A1'] dispense_base = reservoir_well.bottom(DISPENSE_HEIGHT) # --- Tip ordering --- ordered_tips = [] for col in tip_rack.columns(): ordered_tips.extend(col) start_tip_well = tip_rack.wells_by_name()[START_TIP] try: next_tip_index = ordered_tips.index(start_tip_well) except ValueError as exc: raise RuntimeError(f"START_TIP '{START_TIP}' is not in the loaded tip rack.") from exc # ----------------------------------------------------------------------- # MAIN LOOP # ----------------------------------------------------------------------- for color in COLOR_MAP: if not color['points']: continue source_wells = [plate.wells_by_name()[w] for w in color['source_wells']] current_well_index = 0 remaining_volume = WELL_CAPACITY n_dispenses = len(color['points']) protocol.comment( f"--- {color['name']} | {len(source_wells)} wells | " f"{n_dispenses} × {VOLUME_PER_DROP} µL ---" ) # Pick up one tip per color if next_tip_index >= len(ordered_tips): raise RuntimeError("Out of tips for the configured colors.") pipette.pick_up_tip(ordered_tips[next_tip_index]) next_tip_index += 1 for (x_offset, y_offset) in color['points']: # --- Bounds check --- if (abs(x_offset) > RESERVOIR_X_MM / 2) or (abs(y_offset) > RESERVOIR_Y_MM / 2): protocol.comment( f"WARNING: {color['name']} point ({x_offset}, {y_offset}) mm is out " f"of bounds ±{RESERVOIR_X_MM/2:.1f} x ±{RESERVOIR_Y_MM/2:.1f}" ) if not ALLOW_OUT_OF_BOUNDS: continue # --- Switch wells if needed --- if remaining_volume < VOLUME_PER_DROP: current_well_index += 1 if current_well_index >= len(source_wells): raise RuntimeError( f"Ran out of volume for {color['name']} " f"(increase number of source wells)" ) remaining_volume = WELL_CAPACITY protocol.comment( f"Switching to well {color['source_wells'][current_well_index]}" ) source_well = source_wells[current_well_index] # --- Aspirate --- pipette.aspirate(VOLUME_PER_DROP, source_well) remaining_volume -= VOLUME_PER_DROP # --- Move & dispense --- target = dispense_base.move(Point(x=x_offset, y=y_offset, z=0)) pipette.move_to(target) pipette.dispense(VOLUME_PER_DROP, target) # --- Tip handling --- if DROP_TIP_IN_TRASH: pipette.drop_tip() else: pipette.return_tip() Post-Lab QuestionsHugoen