https://pages.htgaa.org/2026a/davi-lima/homework/week-09-hw-cell-free-systems/index.htmlGeneral homework questions 1- Explain the main advantages of cell-free protein synthesis over traditional in vivo methods, specifically in terms of flexibility and control over experimental variables. Name at least two cases where cell-free expression is more beneficial than cell production. In traditional in vivo (inside the cell) methods, the cell’s own survival is the priority. In CFPS, your protein is the priority. You can add non-natural amino acids, chaperones, or labeling molecules directly to the mix without worrying about transport across a cell membrane. You can monitor and tweak variables like pH, temperature, and redox potential in real-time. If a protein is lethal to a living cell (e.g., a pore-forming toxin), CFPS is the only way to produce it because there is no “cell” to kill. You can go from a linear DNA template (PCR product) to a protein in hours, whereas cell production requires days for cloning and transformation.Hugoen