https://pages.htgaa.org/2026a/devorah-wertheimer/homework/week-09-hw-cell-free-systems/index.htmlGeneral Cell-Free Homework Questions Main advantages of cell-free protein synthesis over in vivo methods Cell-free systems offer direct access to the reaction environment — you can adjust pH, redox conditions, cofactor concentrations, and template DNA without having to engineer a living cell to tolerate those changes. You’re also not constrained by what the cell needs to survive; toxic proteins, non-natural amino acids, and unstable intermediates can all be produced because there’s no membrane to cross and no cellular fitness cost.Hugoen