<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 10 HW: Imaging and Measurement :: 2026a-devorah-wertheimer</title><link>https://pages.htgaa.org/2026a/devorah-wertheimer/homework/week-10-hw-imaging-and-measurement/index.html</link><description>Final Project Measurement Plans: Aspects to be Measured:
Please identify at least one (ideally many) aspect(s) of your project that you will measure. It could be the mass or sequence of a protein, the presence, absence, or quantity of a biomarker, etc. What: FAM fluorophore release from cleaved reporter molecules Units: Relative Fluorescence Units (RFU) or fold-change over baseline Purpose: Quantifies Cas12a trans-cleavage activity indicating target DNA presence Range: Expected 1.0× (baseline) to 8-12× (strong positive) fluorescence increase Please describe all of the elements you would like to measure, and furthermore describe how you will perform these measurements. Primary Detection Technology: qPCR Fluorescence Monitoring Instrument: Bio-Rad CFX series or equivalent real-time PCR machine with FAM detection capability Detailed methodology:</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/devorah-wertheimer/homework/week-10-hw-imaging-and-measurement/index.xml" rel="self" type="application/rss+xml"/></channel></rss>