Labs
Lab writeups:
This week we got to spend some time just having fun getting to know the lab space. We made fun petri dish art using colored water, and even got to run some gels (using these fancy machines that dont require any buffers)!
Making the 1X TAE Buffer Prepping the Gel Restriction Digest Running the Gel Results and Imaging Discussion Given that our imaged gel didn’t look like this, we can dive into what might have gone wrong.
This week, we got to utilize an Opentrons liquid handling robot in order to make cool gel artwork! The custom python script tells the Opentrons unit to pick up a pipette tip, when and where to aspirate and dispense, when to switch tips, and when to stop.
Background In this lab, we modifies the color-generatinf chromophore of the purple Acropora millepora chromoprotein in order to create a variety of different colored mutants. Day 1: Preparation of DNA Fragments We performed two PCR reactions, one for the backbone, and one for the color inserts.