Computational Engineering of the MS2 Lysis Protein to Improve Stability, Titers, and Toxicity After reviewing the provided literature on the MS2 lysis protein (L) and discussing the project aims, our group has decided to focus on three interconnected goals:
Goal 1: Increase the stability of the L protein As the “easiest” goal, it is the most computationally tractable. A stabilized protein is less prone to degradation and misfolding, which could directly lead to higher functional titers and serve as a robust starting point for any subsequent engineering.