<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 11 HW: Building Genomes :: 2026a-diogo-custodio</title><link>https://pages.htgaa.org/2026a/diogo-custodio/homework/week-11-hw-building-genomes/index.html</link><description>Part A: The 1,536 Pixel Artwork Canvas | Collective Artwork Colaborated in the stage where a single picture was being formed throughout the 4 plates It was a fun premise to explore could lab automation collaboratively Next year there could be the “constraint” of creating a single image/pattern throughout all the plates to really engage in the collective activity of trying to figure out how to expand on what other people started like a Cadavre Exquis logic Part B: Cell-Free Protein Synthesis | Cell-Free Reagents 1. Referencing the cell-free protein synthesis reaction composition, provide a 1-2 sentence description of what each component’s role is in the cell-free reaction. In a cell-free master mix, the lysate, in this case E. coli, has all the machinery needed to process DNA to RNA to protein. In order for this to work, there is a mix of salts such as Potassium Glutamate, Magnesium Glutamate and Potassium phosphate, which maintain proper ionic concentrations to allow for correct DNA/RNA folding, enzymes to bind, and protein synthesis to occur efficiently, and buffers (HEPES + Potassium phosphate monobasic/dibasic) which maintain the optimal ph conditions for enzymatic activity.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026a/diogo-custodio/homework/week-11-hw-building-genomes/index.xml" rel="self" type="application/rss+xml"/></channel></rss>