Project Description I want to develop a synthetic biology-based point-of-care diagnostic platform that can rapidly detect cholera toxin and toxin co-regulated pilus antigens in stool and environmental samples within 15-30 minutes using engineered cell-free biosensors integrated into a paper-based microfluidic device.
The system employs single-domain antibodies recognition elements fused to split T7 RNA polymerase domains that reconstitute active enzyme only upon antigen binding, driving colorimetric reporter expression for visual result interpretation requiring no laboratory infrastructure or specialized training.
