Wearable Electrochemical Immunosensor for Dual Allergy Detection Abstract Allergic diseases affect over 500 million people worldwide and represent a critical public health challenge. Current allergy diagnostics rely on laboratory-based immunoassays or skin-prick tests that require physical visits to the clinic and specialized equipment, without existence of real-time data during acute exposure events. This project addresses the need by creating a minimally invasive wearable device that simultanesly monitros both histamine, the trigger, and immunoglobin (IgE), antibody for allergic sensation, in real time. The plan is to create two independent, computaitonally designed DNA toehold switch circuits where one switch is triggered by histamine-bound aptamer output and one by IgE-bound aptamer output. The central hypothesis is that two switched can undergoe confromaitonal changes upon target engagement and produce a deetctable signal in a cell-free system.